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After an additional DNase treatment, the absence of remaining DNA traces was confirmed by quantitative PCR (SDS 7700; Applied Biosystems, Framingham, MA) with assays specific for 16S rRNA [39].
After four to six h of stimulation, MDDC were extensively washed with complete medium and the absence of remaining infectious virus particles in the MDDC suspensions was confirmed by plaque assay as described above.
The absence of remaining DNA targets was demonstrated by qPCR analyses (see below) of DNase-treated RNA samples.
CT scan was used in a qualitative way to demonstrate bone bridging of the defects and absence of remaining bone substitute, as an indirect sign of bone remodeling.
The absence of remaining DNA traces was evaluated by quantitative PCR (SDS 7700; Applied Biosystems, Framing-ham, MA) with assays specific for 16s rRNA [ 53, 65].
After additional DNase treatment, the absence of remaining DNA traces was evaluated by quantitative PCR (SDS 7700; Applied Biosystems, Framingham, MA) with assays specific for 16s rRNA and the HU genes [ 32, 70], encoding for a DNA-binding protein.
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The NMR studies on this 142-residue construct reveal that overall subdomain 2 of the HCV helicase is globular and well structured in solution even in the absence of the remaining parts of the NS3 protein.
While the presence or absence of the remaining HSEs varies, this variation is always consistent with the phylogeny.
Although two enzymes of the denitrification pathway (nitrate reduction I) were found, absence of the remaining enzymes is probably the determining factor for the inability of these strains to reduce nitrate to N2, as reported by Dorella et al. (2006).
Thus, the absence of the remaining MITE families in these sequences does not preclude their equally ancient origin.
Likewise, EpICD alone sufficed to substitute for the deficiency to express EpCAM in vitro and supported proliferative signals in the absence of the remaining domains of EpCAM [ 8].
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