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Nevertheless, we must emphasize that the absence of reading modulations via the thalamus, in the global analysis, may indicate that the thalamic pathway was not selective for reading.
Reading difficulties were the primary concern of the children and their parents and no subjects reported writing difficulties in the absence of reading difficulties.
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Following this, we checked for coverage uniformity to ensure absence of read depth bias in 3′ or 5′ positions by calculating read coverage for each nucleotide using geneBodyCoverage.py module in RseQC.
When we looked at the AP2-exp locus, instead of an expected absence of reads due to the disruption of the gene and absence of a stable 3′ UTR, we observed more uniquely-mapped reads that could be aligned to the region corresponding to the first homology box designed for integration in the AP2-exp locus in the mutant line than in the wild-type.
The last false negative was missed due to absence of reads mapping to the site.
Complete absence of reads mapping to exon 8 is indicative of a homozygous deletion.
Missing values for RH indicate the absence of reads supporting the alternative isoform.
An almost complete absence of reads for the mitochondrial genome supports the efficacy of the macronuclear purification.
8,072 of such bins were characterized by either the presence or the absence of reads (P/A regions).
Therefore, the absence of reads that overlap with the sequences of the cosmid termini pointed to a deficiency of breaks near to the ends of the linearized DNA.
However, this absence of reads is within sampling error based on the RNA-seq coverage (225×) given transcribed allelic fraction of vIII at 1.6%% as estimated by NS.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com