(A ) Summary plots of ChIP-seq values for binding sites belonging to cluster D. The regions were divided based on the presence or absence of annotated ESC enhancers (Creyghton et al., 2010 ; Whyte et al., 2013 ).
The binding sites of cluster D (excluding regions with TSSs) were divided into two basic groups based on the presence or absence of known ESC enhancers (Creyghton et al., 2010 ; Whyte et al., 2013 ).
Thus, in the absence of Esrrb, ESCs lose the ability to respond effectively to Gsk3 inhibition and their propagation appears strictly dependent on LIF.
Corresponding to the absence of resistance to ESCs, no penA mosaic alleles or A501-altered PBP2 amino acid sequences were found in any of the isolates.
mRNA expression analysis of the PRNP-related proteins PRND (Doppel) and SPRN (Shadoo), whose PRNP function is known to be redundant, revealed their incapacity to compensate for the absence of PRNP during early ESC differentiation.
We tested the capacity of forced E-cadherin expression to stabilize ESC self-renewal in the absence of Oct4 using a ZHBTc4 ESC line harboring an inducible E-cadherin protein.
Embryoid bodies are formed "in vitro" after several days of growth of ESC in the absence of LIF (leukemia inhibitory factor).
Interestingly, compared to the absence of progerin in HGPS-ESCs, differentiation into MSCs resulted in the re-expression of progerin at early passages and much more accumulation at late passages (Fig. 4E and 4F).
ICM and ES cells seem therefore to be more closely epigenetically related to each another than ES and TE cells, an observation in line with the absence of similarity of mouse ESCs with cells of the TE [7].
The correlation between NANOG and Klf4 was abrogated in the absence of pSTAT3 in Stat3+/+ ESCs without LIF and in Stat3−/− ESCs, showing that NANOG-driven Klf4 upregulation was pSTAT3 dependent.
At cellular level it enhances proliferation and self-renewal of ESCs in the absence of leukemia inhibitory factor (LIF).
