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hMSC transfected with miR-320c or control miRNA were cultured in presence or absence of AIM, total RNA was extracted from baseline and following 7 days of AD induction and subjected to microarray analysis.
The 3T3-L1 cells transfected with the synthetic miRNA mimics or inhibitors were cultured in six-well plates for 3 days in the presence or absence of AIM (2/3 concentration for stimulatory studies or full concentration for inhibitory studies) as mentioned above followed by treatment of insulin alone for one more day.
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The cells were then cultured in the presence or absence of AIMs (400 μg/ml) in serum-free media for 24 h.
While it is important to delineate RECs in terms of competence in order to maintain the highest standards of health research in SA, in the absence of redress aimed at raising the level of functioning of RECs at historically disadvantaged institutions, the NHREC system of accreditation will unintentionally serve to perpetuate the injustices of apartheid.
In the absence of this, the aim is to "manufacture some sort of political paralysis while at the same time raising the level of pro-Russian voices" to "destabilize politics and sow chaos," she said.
A comparison of plant growth-promoting factors, namely IAA, ammonia, phosphate solubilization and HCN production, was quantitatively performed in the presence and absence of chicken feathers, aiming to evaluate any positive and negative role of feather metabolism in these attributes.
However, in the absence of specific components aimed to raise employability, these types of ALMPs could result in wage moderation and even in an increase in the number of working poor (Clasen and Clegg 2006).
The absence of the cap aims to represent "Shakira's bare feet".
[48] [50] Arguments regarding the effect of retinal neurodegeneration caused by diabetes on retinal thickness parameters are scientifically interesting, but, in the absence of specific therapies aimed at this neurodegeneration, are of mostly academic interest.
Therefore, in the absence of a precise aiming device not all screws were exactly cut in the middle of the longitudinal axis of the screws.
For example the well-established multidisciplinary format of cancer care involves relevant professions meeting in the absence of the patient aiming to facilitate unbiased discussion [ 31].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com