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Ultrathin sections (70 nm) were cut as above, transferred onto formvar-coated copper grids and stained with lead citrate, uranyl acetate and 1% phosphotungstic acid.

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3. Next mix together the ingredients for the tomato frittata, using 3 eggs instead of 4 if desired, and proceed as above, transferring the mixture to the breadpan when it has reached the consistency of runny scrambled eggs and spreading in an even layer on top of the onion and zucchini layer.

As mentioned above, transfer events of ribosomal genes enhanced the competence of G-plasma and greatly influenced the community structure in AMD.

After following the steps above, transfer the prepared macaroni into a large, oven-safe glass or metal casserole dish (or two, if you don't have a large enough dish).

Gas chromatographic conditions were as reported above; transfer line temperature, 295°C.

Briefly, undifferentiated HESC were harvested with 1 mg/ml collagenase IV as above, pelleted by centrifugation and resuspended in HESC medium containing all supplements as described above, and transferred to sterile 10 cm bacteriological Petri dishes (Sterilin, UK).

All parameters value described above were transferred into digital format using the Arc GIS software.

Induced MSCs were harvested with the mixture of enzymes mentioned above and transferred to sterile slides for cellular adherence.

The conjugate was purified by repeated centrifugation as described above and transferred to 0.25 M ammonium acetate buffer, pH 5.3.

We purified and PKH67 labelled CD8β+ T cells from MCM1 as described above and transferred them into MCM2.

Workers were collected from the colonies, as described above, and transferred to plastic tubes (diameter 6 cm, height 12 cm) where each individual was kept isolated.

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