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Contigs were locally assembled for each RAD sequence with Velvet using data from both libraries and the same processing as above to remove over-abundant reads.

A chloroform extraction was performed as above to remove RNaseA and any additional contaminants.

PCR product was size-selected as above to remove unreacted primer and adapter dimers.

The ribosome complex was then pelleted through a sucrose cushion as above to remove GTP and excess eEF2 and resuspended in the same buffer.

The data set was reduced as above to remove additional read-pairs mapping in the same location leaving 2,808 Class 2 events that were indicated by a single read-pair.

Saturated ammonium sulfate (AS) was added to reach 20%and19%9% of saturation, to the soluble- and wall-bound fractions, respectively, stirred for 30 minutes at 4°C, and centrifuged as above to remove PVP from solution.

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Update 7/12/07: Alex Needham (arts blog acting editor writes): As Tim Jonze disputes Morrissey's account of the "David Bowie" and "Knightsbridge" exchanges, I've edited the above post to remove them.

Ultrasonication was used in combination with above solvents to remove the impurities, and then dried in vacuum at 40 °C.

Then repeat the previous steps and delete the "Pokki Download Helper" folder using the same method as above to completely remove Pokki from your system.

The silver nanoparticles formed by all four protocols described above were purified to remove the excess enzyme and other soluble impurities using ultracentrifugal filters (Amicon® Ultracentrifugal filters by Millipore Corporation (Billerica, MA, USA) having molecular weight cut-off value of 30 kDa operating at 2,000 rpm for 15 min).

As above, this suggests to remove an interaction, namely the regulation of GAL80 by Swi5.

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