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The initial testing for time- and dose- dependent effects showed that the above tissue and substrate concentrations, and time of incubation were optimal for metabolism of 7DHC and 7DHP (not shown).
Volumes contained in the bounding surface (called 'anatomical volume'), as well as that identified as containing any of the above tissue categories (called 'morphological volume'), were calculated.
Five representative areas were taken per mouse and analyzed using Image J. To evaluate epidermal thickness, the above tissue sections stained with hematoxylin and eosin (4X magnification images) were analyzed using Image J.
Some silent genes had already been detected as jiDE genes by SNEP analysis, however, further silent genes were detected by the above tissue expression profiling analysis, and added to the set of jiDE genes.
In the study of NPWT on free flaps discussed above, tissue pressure was monitored at two locations: at the interface between the flap and the device (white PVA foam) and deeper at the level of the vascular anastomosis.
The above tissue array sections were prepared for immunohistochemistry of NPM with a three-step immunoperoxidase method using a Strept-Avidin Biotin kit (Dakopatts, Glostrup, Denmark) as previously described (Yun et al, 2003, 2004).
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Histological examination showed systemic inflammatory reactions in the above tissues, with hypertrophied nuclei and phagocytic cell formation, hemocyte aggregation, epithelial cell shedding, vacuolar degeneration and focal necrosis.
Repeated injections were performed at 1, 3 and 5 days post-injury; with harvesting of the above tissues performed at 7 days post-injury.
Real-time PCR was introduced to detect the mRNA levels of the different proteins in the above tissues to confirm the uniform co-expression of the four fluorescent proteins further.
Cluster analysis allowed us to identify subgroups of miRNAs enriched in each of the above tissues.
No positive staining was detected in the above tissues of vaccinated pigs– 5(h)).
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