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Place the contigs one-by-one based on the above sorted list starting from C 1 ′ For any contig C, mappings for this contig will be considered as per the list L C. In other words, the first time when we try to place C, we will use the mapping found in L C [ 1].
The above sorted MAGI-CCR-5 cells were analyzed for C46 expression by trypsinizing 1×106 cells and washing twice with Dulbecco's modified phosphate buffered saline (D-PBS), then blocking in D-PBS with 5% nonfat milk on ice for 10 minutes.
If you have everything above sorted out and your tank appears to be in good working order, fill it to the top with water.
Cells were labeled with Hoechst 33342 as indicated above, sorted with a FACSvantage flow cytometry system (BD Biosciences) according to their cell cycle phase; G0/G1, S, or G2/M, collected at 4°C and lysed immediately after sorting.
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Guatemala and Honduras had the biggest gaps in height between rich and poor women; Uganda and Ethiopia (above, where women sorted coffee) had the smallest.
In vivo infected pigs were euthanized 10 days post-infection, their lung collected and BAL and parenchymal cells extracted, stained, analysed and sorted as above.
In vivo infected pigs were euthanized 10 days post-infection, their lung sampled and BAL and parenchymal cells collected, stained, analysed and sorted as above.
Each node is visited sequentially while checking against all nodes above it in the sorted array.
On the basis of these sorted output values from the above four procedures, each molecule would then receive a binary 1.0 vote for every 'top 10% appearance'.
Approximately 3 × 106 cells in each phase of the cell cycle were sorted by FACS, and DNA was extracted from the sorted cells as described above.
For in vitro gene expression assays, MPs, MDPs, and CDPs were sorted as described above.
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