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Next, we analyzed polarization above rigor noise.
► During contraction, bimodal Gaussian photon distributions were observed above rigor noise.
The additional two modes above rigor (see Fig. 5) under contraction conditions were significantly greater by magnitude (photon counts) and numbers.
The fact that contraction above rigor modes can be described by two Gaussian and rigor cannot is significant because it suggests that the two Gaussians represent pre- and post-power stroke conformations.
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McNutt hopes the retraction will also help curb what she sees as a rush-to-publish mentality among scientists that puts staking a claim above scientific rigor.
During contraction, in contrast, bimodal Gaussian photon distributions were observed above the rigor background threshold.
Second, the acquisition of photon distribution above biological rigor noise via our threshold algorithm became necessary because of the overlapping signals in time.
Our finding that the contraction data showed intensity signals in two polarization-sensitive detection channels with the two mean maxima above the rigor fluctuations has major implications.
Fig. 6A shows the histogram of measured time-depended polarized fluorescence intensity fluctuations above the rigor background threshold of 5.0 KHz in Ch1 and 6.4 KHz in Ch2 for that particular motor molecule under contraction.
All contraction data showed the presence of additional dynamic modes in the orientation motion of actin filaments above the rigor fluctuations in intensity signal in two polarization selective detection channels (see Figs. 4 and 5).
By the time Williams was discovered by his personal assistant, suspended in a seated position above the floor, rigor mortis had already set in.
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