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Slides were washed as described above, mounted using Prolong Gold Antifade (P36930; Life Technologies) and shielded from light.
They are constructed using thin glass slides (covered with poly-L-lysine; see above) mounted with silicon fat (Baysilone-Paste, mean viscosity; Bayer, Leverkusen, FRG) at the bottom side of a conventional 35 mm dish in which a hole was drilled.
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The enterprise version of the drone runs $2,000 — a price that includes the Mavic 2, remote, battery, the above mount accessories and a case.
Tissues were air dried as above and mounted with cover slips.
The samples were washed as above and mounted in UltraCruz mounting media containing DAPI (Santa Cruz, sc-24941).
Sections were washed in TBST as above and mounted with fluorescent Vectashield with 4', 6-diamidino-2-phenylindole (DAPI).
F-actin was stained as described above and mounted on slides cover with ProLong solution (Invitrogen).
Sections were dehydrated (as described above) and mounted using Permount (Fisher).
In brief, rats were anesthetized as above and mounted on a stereotaxic apparatus and fixed accordingly.
Sections from adult animals, stained as described above, were mounted on coated slides, dehydrated and cover slipped.
Gametophytes were fixed in the same way as commented above, and mounted on microscope slides for gender examination.
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CEO of Professional Science Editing for Scientists @ prosciediting.com