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Receptor binding experiments were done on the membranes obtained from the above mentioned cells using 3H-spiperone as radioligand.
However, like photoreceptor precursor and retinal progenitor cells, issues pertaining to host donor compatibility and ethics of cellular isolation exist (i.e. human versions of the above mentioned cells are isolated during embryonic development).
In view of the above reports, the wound contraction and healing effects of P. lappacea might be attributed to its ability to stimulate and increase the synthesis of one or more of the above mentioned cells and substances/factors known to promote wound healing.
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On each set of arrays also the above mentioned cell lines and 2 bridge samples were printed for antibody signal normalization between the 2 sets.
As demonstrated using the above mentioned cell types, production of iPSC derived retinal cells expressing a variety of retinal proteins, including those specific to photoreceptors, have been achieved [21], [22].
For each patient (n = 4), two pellets were generated using each of the above mentioned cell concentrations.
See Additional file 1 for the original data that was used to determine the above mentioned cell properties.
Raman excitation close to the edge of RBC allowed us to get signals from the above mentioned cell's regions which undergo maximum deformation.
As per the protocol, BM received after the initial five doses of chemotherapy, showed an abrupt reduction in the percentage of the above mentioned cell population.
All the above mentioned cell lines were maintained in high glucose Dulbecco's Modified Eagle Medium (DMEM) (Life Technologies, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS) (Life Technologies).
So far all cell lines analyzed were found to be essentially negative for endogenous DC-SCRIPT mRNA expression, including the above mentioned cell lines and 36 other breast carcinoma cell lines (data not shown).
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