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Pooled peak fractions were concentrated as above, frozen in liquid N2, and stored at −80 °C.
Fractions containing SeCitS were pooled, concentrated as above, frozen in liquid nitrogen and stored at -80°C.
The brains were cryoprotected in 30% sucrose as described above, frozen in crushed CO2, and cut into sagittal or coronal, 18 μm thick, cryostat sections.
The samples were collected at 10, 20, 30, 60, 120, and 180 min. The medium was removed, and cells were washed with 1× PBS and then scraped into 400 μL of 20 80 phosphoric acid/acetic acid as described above, frozen in dry ice, and kept at −80 °C before thawing for catechol assays.
Similar(56)
Temperature just above freezing.
The temperature was just above freezing.
The dawning sky was clear, the temperature just above freezing.
Southerly airstreams occasionally bring days with temperatures above freezing.
At that protest, however, the temperature was above freezing.
Even so, her temperature barely climbs above freezing.
The situation is all the more urgent given night-time temperatures that hover just above freezing.
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