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After the preparation of a concentrated stock solution, the above concentrations (0.1 1 mM) were obtained by corresponding dilutions.
The results showed that all the extract and fractions that induced cell toxicities were negligible at the above concentrations [16].
To study the neuroprotective effect of PTE against H2O2 cytotoxicity, the dIMR32 cells were pretreated for 24 h with the above concentrations of PTE, and then treated with the 300 μM of H2O2 for 24 h.
In contrast Wortmanin at both the above concentrations clearly decreased the levels of nuclear HSF1 (Fig 2B).
However, plumbagin did not exhibit any significant toxicity on normal cells (PBMC) at any of the above concentrations, suggesting that plumbagin possesses selectivity between normal and cancer cells.
For internal application, the reagent solution was placed in the patch pipette at the above concentrations and the tip back-filled with pipette solution devoid of reagent.
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Comparatively, the pH 4 for the above concentration has the most intense and sharp peak.
Inhibition study was conducted by adding the above concentration at the start of culture.
PA in the above concentration range did not scavenge DPPH radical (P > 0.05).
The above concentration ranges correspond to the same molarities of 0.1 200 μg/mL mAb protein in plasma.
The objective was to maintain serum glucose concentrations between 7.0 and 10.0 mmol/l, which is well above concentration range of 4.4 6.1 mmol/l referred to above.
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