Sentence examples for above blocked from inspiring English sources

Sections were retreated with citrate as above, blocked 5% normal swine serum (Jackson) and labeled for 3 hours with c-KIT specific antibody followed by labeling with a AP-conjugated secondary antibody.

Sections were prepared as above, blocked with 20% nonimmune serum, and incubated sequentially with rabbit anti-CLR antibody (1 3000), rabbit anti-CD31 antibody (1 1000) (Abcam, Cambridge, UK), and sheep antihuman podoplanin (1 500) (R&D Systems, Abingdon, UK), for 18 h at 4 C. Control sections were incubated with an equivalent concentration of normal IgG or nonimmune serum.

For simultaneous detection of tubulin and DNA with mouse monoclonal antibodies, we stained first for DNA as detailed above, blocked the secondary antibodies with mouse normal serum, and stained with a directly FITC-labelled tubulin antibody (DM1A; Sigma) at a 1 100 dilution.

Six h after administration of 10  μg labeled OVA, the NALT cells and the nasal passage cells were prepared from the mice as described above, blocked with FcR blocker (Milteny Biotec, Bergisch Gladbach, Germany), stained with phycoerythrin- (PE-) labeled anti-mouse CD11c antibody (eBioscience, San Diego, CA), and subjected to FCM analysis.

The cells were then stained with Rho1D4 monoclonal antibody diluted 1∶1000 in the above blocking buffer for 1 hour.

In all, 10 n M SW 120 was then added to the cell culture dishes containing the above blocking compounds.

As discussed above, blocking pDC production of IFNα was found to prevent spontaneous development of psoriasis in xenograft transplant mouse models (Nestle et al., 2005).

Membranes were subsequently incubated with rabbit anti-human Panx1 1:250 (Novex, Life Technologies) and rabbit anti-human Cx43 1:6000 (Abcam) in the above blocking buffer overnight at 4ºC.

After being labeled overnight at 4°C with anti-serotonin (diluted 1 10,000 in the above blocking solution), specimens were washed in PBS and reacted for 1 hour (cryostat sections) or 3 hours (whole-mounts) with Cy3-conjugated goat anti-rabbit-IgG.

However, in this paper, the support has finite cardinality, which makes the above block inapplicable.

Co-deposition of the above blocks in a solvent-free environment results in the controlled assembling of crystalline organometallic products.