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The frequency of aberrations was evaluated by counting the percentage of cells (mitoses) positive for one or more of the above aberrations.
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As mentioned above, these aberrations may be correlated and therefore not independent: this will reduce their impact on the σ/ I values.
All other lesions listed above were considered structural aberrations.
To explore in vitro drug sensitivities of cell lines expressing the various molecular aberrations highlighted above, a panel of 24 GC cell lines were exposed to drugs targeting FGFR2 (PD173074, FGFR2 phosphorylation IC50 ∼10 n M) (Kunii et al, 2008), HER2 (Lapatinib, HER2 enzyme IC50 9.2 n M) (Rusnak et al, 2007) and MET (crizotinib, MET enzyme IC50 6 n M) (Timofeevski et al, 2009).
We also provide estimates of the number of cell equivalents that need to be scored to detect a given increase in aberrations above the background level surveyed in this population.
Finally, in the regime determined above, where known aberrations could be reliably measured (200 µm depth for the 63×/0.9 objective, 400 µm depth for the 20×/0.5 objective), PSFs are calculated from the wavefront measured by CGWS using the first 28 Zernike coefficients whose tilt and defocus are numerically removed (Fig. 7 ).
Figure 4 shows the application of the above method for measuring residual aberrations to a focal series reconstruction of the Si(_3 N _4) sample.
"When you put profits above patients," he said, "aberrations like this happen".
The algorithm for measuring and correcting residual wave aberrations described above requires a relatively flat, defect-free region within a portion of the full field-of-view.
The aberrations mentioned above lead in turn to karyotypic profiles which differ in various subtypes of melanoma.
However, all MTF data calculated and discussed above include all optical aberrations and thus represent how rodents naturally see.
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Justyna Jupowicz-Kozak
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