Exact(4)
Grinding-wheel cutters have a planetary motion, rotating rapidly about their own axes, which in turn slowly rotate with the boring head about the spindle axis; the hole diameter is controlled by adjusting the distance between the wheel axis and the spindle axis.
Thus, a systematic representation of the vast existing knowledge about the spindle checkpoint and the role that phosphorylation plays in it would be a valuable tool for gaining further insights into the process.
Despite the large mass of quantitative data known about the spindle assembly checkpoint, we have seen that the models developed so far have had a limited impact because of the lack of specific experimental data.
However, for those where sister separation was observed in MII, the newly formed single chromatids oscillated about the spindle equator, presumably because they have only a single kinetochore that fails to establish simultaneous attachment to both spindle poles (Fig. 7E; supplementary material Movie 2).
Similar(56)
Although checkpoint activation is well characterized, far less is understood about how the spindle checkpoint is silenced when all sister chromatids are attached to spindle microtubules in an appropriate fashion.
This paper presents an overview of the research about the compensation of the spindle thermal error.
In the other cases, sister chromatids were lagging behind on the spindle during anaphase, with about two-thirds eventually moving to the correct and the remainder moving toward the incorrect pole.
Information about the human spindle checkpoint proteins BUB1 and BUB1B, and their yeast homologs BUB1 and MAD3 was captured through a combination of text and data mining approaches.
When checking anaphase spindles 10 min after the release, both cen2-GFP marks were found close to one edge of the spindle in about 20% of cells.
In contrast to asymmetric division, we know much less about the mechanisms that position the spindle during symmetric cell divisions.
We found that even before anaphase, alignment of chromosomes on the mitotic spindle was defective, since the cen2-GFP mark was found close to one of the spindle poles in about 60% of the ark1-T7 cells at restrictive temperature.
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