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The fact that over-expression of the Wnt antagonist, Dkk1, in transgenic mice is able to abolish the expression of a number of perivenously expressed genes argues for the existence of a physiological signal delivered to perivenous hepatocytes by agonistic Wnt molecules (Benhamouche et al. 2006).
To that end, we used siRNA to abolish the expression of CREB in HAEC.
Accordingly, mutations in rhi abolish the expression of these loci, which results in a general depletion of the corresponding piRNA population (Klattenhoff et al, 2009).
A set of these regulators will usually act in coordination to dampen or abolish the expression of a common target, although a single miRNA may efficiently silence a gene [ 3].
This is evidenced by the increase in HMOX1 mRNA levels observed in TFEB overexpressing cells treated with sucrose and by the fact that GSH did not completely abolish the expression of HMOX1 after long exposure to Cu.
In K. lactis, RAG5 mutations [ 38] abolish the expression of RAG1 for a low-affinity glucose transporter [ 39] and decrease the level of the 2.0-kb mRNA species of HGT1 for a high-affinity glucose transporter [ 40].
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Interestingly, knockdown of HOIP abolished the expression of these cytokines in cells expressing TRAF2-5KR, but not wild type TRAF2.
This polymorphism selectively abolishes the expression of ACKR1 in erythroid cells, causing a Duffy-negative phenotype.
In a separate cohort, the mGlu2/3 agonist LY354740 (10 mg/kg), given prior to the EtOH challenge, abolished the expression of sensitization.
Gonadectomy lowers dramatically the circulating levels of androgens measured at 4 and 8 days post-castration and abolishes the expression of nest building, courtship behavior and nuptial coloration, but has no effect on the expression of aggressive behavior.
Interestingly, three consecutive rare codons placed in ORF1 effectively abolished the expression of mCherry.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com