For this purpose, we selected the cores showing ligand (IGFI or IGFII) expression and satisfying all of the control staining tests for the expression of the two receptors (IGF-IR and IGF-IIR) to be able to evaluate the proportion of cores showing receptor expression.
As HT1080lacZ-K15 cells do not endogeneously express L1CAM (Fig. 3A), we were able to evaluate the impact of FL-L1CAM and SV-L1CAM on metastasis formation without interference through expression of the other isoform.
By comparing estimated rates of molecular substitution, codon usage, and gene expression in these species with those of Arabidopsis, we were able to evaluate the role of positive and relaxed selection in driving the evolution of Cardamine genes.
With rapid progress in mapping regulatory elements and the growing availability of cell and tissue-specific gene expression data, future studies should be able to evaluate the functional relevance of genetic variants at 9p21.3.
Finally, the present study was not able to evaluate the histological patterns, molecular markers (such as the expression of estrogen receptor) or clinical stages of breast cancer.
Randomised control trials are effective at evaluating an intervention as a whole, but are not able to evaluate the independent or interactive effect of intervention components24.
Are they able to evaluate the difference?
For some of the knockouts we were able to evaluate model predictions with available gene expression data from transcriptome studies.
Further, benchmarking is only able to evaluate methods implemented in a current release of the software.
By taking advantage of these mice in future studies, we will be able to evaluate localization patterns within specific neuron populations and subcellular fractions to determine if these factors contribute to the expression of specific isoforms.
We were able to evaluate inositol-mediated gene expression changes because of our simultaneous analysis of expression changes in Li-treated mice and in mice harboring KO of either IMPA1 or Slc5a3, both encoding proteins related to the PI cycle and shown to be affected by therapeutic levels of the drug.
