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The ability to perform multiplex-ready PCR under standardised reaction conditions enables any combination of markers to be deployed for multiplexed amplification, and can improve assay throughput by facilitating PCR automation.
A major improvement of LIPOchip, versions 7 and 9, is the ability to detect copy number variation (deletions or duplications of entire exons) in LDLR, thus abolishing the need to perform multiplex ligase-dependent probe amplification in patients with FH.
Antibody arrays are a relatively new technology that enables one to perform multiplex high-throughput protein expression profiling.
This system could use a single enzyme as label to perform multiplex immunoassay in one fluid way.
A more recent protocol, although providing the ability to perform precapture multiplexing on both solid-phase and solution-based TGE is optimized for SOLiD sequencing [ 11].
We used our nested MSP assay, described in detail previously (Palmisano et al, 2000), because of its increased sensitivity for the detection of promoter hypermethylation in biological fluids and because of the ability to perform Stage 1 multiplex PCR.
A practical advantage of multiplex-ready PCR is the ability to perform assays under standardised conditions.
Although reagents for multiplex and real time PCR assays are traditionally considered expensive, the ability to perform these assays within a short time frame to detect multiple pathogens reduces hands-on time in the laboratory, is more efficient and can generate valuable information in differential diagnosis.
His ability to perform skilful balancing acts was impressive.
The ability to perform self-behaviour analysis.
On their ability to perform?
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