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As predicted, it is impaired in its ability to interact with eIF4GI (Fig. S6).
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Our experimental data on protein interaction show a reduced ability to interact with PDE4A5 supporting the possible functional impact of the variant.
Final compounds were evaluated in radioligand binding assays for their ability to interact with 5-HT6 receptors.
These data support the notion that the ability to interact with Beclin1 and regulate autophagy may be linked to the PLP2-TM control of innate immune signaling.
ScPrp17 N-terminal sequences, but not its WD40 repeats, conferred the ability to interact with ScPrp19 (Figure S2A).
Therefore, we have selected a small osmotin peptide (peptideOSM) and evaluated its ability to interact with ADIPOR1 and PHO36.
Interestingly, Swi3-E68 (K47N, Y111C) retained the ability to interact with Swi1 (Figure 2A), and corresponding mutant cells were sensitive to CPT, but not HU and MMS (Figure 1A).
This resulted in markedly reduced purification of intact eIF4G and corresponding precipitation of ∼100 kDa N-terminal fragments that retain the ability to interact with eIF4E (Figure 1D).
Both of the Sox17 truncations and the point mutant maintained the ability to interact with Smad3 across multiple binding stringencies (Fig. 7C).
To investigate whether Ynd1 mutants mediating the E4orf4 toxic signal, and specifically the cytosolic mutant, have retained the ability to interact with E4orf4, coimmunoprecipitation experiments were carried out.
As shown in Fig. 5B, neither deletion of the BH4 domain nor the Y101K mutation affected Bcl-xL's ability to interact with Praf2.
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