In order to compare the ability of assembling repeats fairly, all contigs are aligned back to repeats using swalign function [ 25].
Many of the functional attributes of pectin, whether in the plant cell wall or in engineered food materials, are linked to its gelling properties and in particular to its ability to assemble in the presence of calcium.
Regarding the first application, the ability to assemble in one step a construct from 10 different plasmids should allow much more flexibility and efficiency in making constructs than is now possible.
However, both NOMAD and the various BioBrick standards are limited in their ability to assemble multiple DNA fragments in a single step, and still rely on procedures that limit their potential for automation such as extraction of DNA fragments from gels or the requirement for the design of custom primers for specific constructs [13].
However, the hemagglutination assay and antibiotic sensitivity assay are screening tools, and as such, they lack in sensitivity to pick up smaller changes in the ability to assemble pili or channel activity of the usher.
Additionally one should keep in mind that the hemagglutination assay and antibiotic sensitivity assay (used in this study) are screening tools, and as such, lack in sensitivity to pick up smaller changes in the ability to assemble pili or channel activity of the usher.
Our experiments show that the insert length and in particular the variability in the insert length play a crucial role in our ability to assemble haplotypes.
The chaplin proteins are remarkable in their ability to assemble rapidly on agitation.
Mutants that are compromised in their ability to assemble functional carboxysomes have a high-CO2-requiring (hcr) phenotype and do not grow at all or at greatly reduced rates, unless cultured in an atmosphere of elevated CO2.
The fact that minor perturbation of final insulin structure caused by G(B23)V substitution is greatly outweighed by major defects in the ability to assemble the insulin chains with proper cysteine pairings indicates that this MIDY mutant is blocked primarily in the protein folding pathway rather than in the stability of the insulin native state.
The utility of Boolean gating analysis lies not only in the ability to assemble a detailed view of responding CD8+ T-cell populations (Fig. 5), but also in the opportunity to look within selected response categories for relationships between responses and clinical status.
