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These data correlate with results of DNA methyl-accepting ability assays showing a widespread hypomethylation of DNA extracted from cells over-expressing PARG (Figures 6B).
Cell viability, colony formation and anchorage-independent growth ability assays were performed to examine cell proliferative capacity and tumorigenicity.
In the mouse A17 cell model previously related to mesenchymal cancer stem cells and basal-like breast cancer, we biochemically dissected the signaling pathways involved and performed functional in vivo tumor growth ability assays.
An unpaired Student's t-test was used to determine significance for gene expression, paraquat sensitivity and climbing ability assays (GraphPad).
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Both DPPH and the ferric reducing ability assay (FRAP) were used to evaluate the same propolis extracts from the south of Brazil.
Given that the biologically correct assay (i.e. fertilization ability assayed with human oocytes) was not possible for obvious ethical reasons, two different approaches were employed.
A subline designated as P4 was also established by selecting clones that exhibited enhanced migratory/invasive ability assayed by transwell chamber and wound migration [18].
Alveolar macrophages were evaluated in terms of macrophage spreading ability assay.
To investigate the role of CPE expression on the tumorigenic activity of CRC cells, anchorage-independent growth ability assay was performed.
Additional studies in long-term dapsone therapy leprosy patients using a ferric-reducing ability assay demonstrated increased antioxidative capacity in erythrocytes.
Concerning chelating ability assay, these two purified quinochalcones appeared to be better chelators of ferrous irons than the positive control EDTA (IC50 = 100.00 ± 0.01) with IC50 ranging from 9.23 ± 0.29 to 12.33 ± 0.25 μg/mL.
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