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The p-values for the regulatory abilities were calculated under the null hypothesis H0 : b ik = 0 or H0 : a ij = 0 [ 30].
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The encapsulation efficiency and thermal storage ability were calculated by the extracted data from DSC analysis for all the samples and their thermal conductivities were measured too.
In this in vitro study, the core (gutta-percha or gutta-percha and carrier)/sealer ratio and the sealing ability were calculated for 4 different gutta-percha techniques: Thermafil, JS Quick-Fill, System B, and lateral condensation.
The ·O2- scavenging ability was calculated as: Δ A 325 nm, control T - Δ A 325 nm, sample T / Δ A 325 nm, control T × 100 % (9).
The O2– scavenging ability was calculated as: Inhibition % = A 325 nm, control T − A 325 nm, sample T A 325 nm, control T × 100%%.
Radical scavenging ability was calculated by a linear regression analysis among the inhibition percentages against the compounds concentrations via the Excel program.
Hydrogen per oxide scavenging ability was calculated by following equation: Hydrogen peroxide scavenging activity = 1 – absorbance of sample / absorbance of control × 100 (7).
DPPH free radical scavenging ability was calculated by using the following formula: Inhibition % = (1 – A/A0) × 100 where A0 is the absorbance at 517 nm of negative control, and A is the absorbance of mixture containing DPPH and sample.
The DPPH radical-inhibiting capacity (radical scavenging ability) was calculated using the following formula: {text{Inhibition}};left( {{text{scavenging}};{text{activity}}} right)% = left[ {left( {{text{A}}_{text{C}} - {text{ A}}_{text{S}} } right)/{text{A}}_{text{C}} } right] times 100 (1)where AC = absorbance of the DPPH solution, AS = absorbance of the sample.
For each reduced-length sequence set, species discrimination ability was calculated.
Defined change in work ability was calculated from the work ability score of the WAI, [ 11].
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