Here's how Mr. Raffles describes what Mr. Glick observed: the spiders "not only climb up to an exposed site (a twig or a flower, for instance), stand on tiptoe, raise their abdomen, test the atmosphere, throw out silk filaments, and launch themselves into the blue, all free legs spread eagled, but they also use their bodies and their silk to control their descent and the location of their landing".
Swinging and/or percussion ausculatation on both sides of the abdomen, tests for a reticular foreign body and rectal palpation were also carried out.
The abdomen was tested on the midline at the midpoint between umbilicus and pubis.
We therefore also generated null mitotic clones of abd-A and abd-B in the developing pupal abdomen to test whether Hox proteins are required to maintain tsh expression during pupation.
For abdominal withdrawal latency (AWL), the abdomens of tested rats were shaved and a protocol similar to the one described for PWL was adopted.
We consider six check-ups and advice: weighing, blood pressure, blood test, urine test, abdomen check-up and ultrasound test, advice on food and personal care.
A similar protocol was adopted in testing mechanical hyperalgesia on the abdomen.
Similar mechanisms exist in other insect systems [41] [43], and abdomen distension has been tested in honey bees [44], but it remains unclear if abdominal enlargement is a physiological mechanism for reproductive senescence in queen bees.
DNA was extracted from the thorax and abdomen of the males tested (after storage in 96% ethanol at -20°C), with the commercial prepGeM kit (ZyGeM Ltd ,Hamilton, New Zealand).
Quantitative RT-PCR was conducted for some genes using the same tissues tested (head, abdomen, thoracopods and photophores) to confirm the integrity and robustness of EST sequencing.
