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Approximately 150 μl of Agrobacterium culture was infiltrated at four points into a young leaf.
At approximately 2 4 weeks or 2 3 true leaf stage, a young leaf was sampled for DNA extraction and genotyping.
A leaf disc (an approximately 5 mm square) excised from a young leaf of a Spinacia plant (from germplasm accessions and cultivars) was used for each measurement.
Pyrosequencing of a young leaf cDNA library generated a total of 111,814 high quality reads, with an average length of 447 bp.
For all germinated accessions, a young leaf sample of approximately 2 cm was ground in 800 μL of preheated (65°C) extraction buffer [ 32].
However, these authors neglected to discuss the data presented in their Figure 3b (lanes 2 and 3), which showed a seemingly greater amount of cpDNA in a young leaf than a mature leaf.
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Curled tightly, as if in slumber, a young leaf-cutter bee plucked from the tar looks ready to emerge from its nest and embark on a full life of pollination and leaf-cutting.
DNA was extracted from a single young leaf from each plant, using a phenol-chloroform extraction method [ 48].
For leaf cell hydraulic conductivity (Lplc) measurements, a mature young leaf blade (still attached to the plant) was fixed onto a metal sledge.
Total DNA was extracted using the REDExtract-N-Amp™ Plant PCR kit (Sigma-Aldrich Corp., MO, USA) from ∼ 2 mm of a healthy, young leaf per sample.
For the NS population, M1 seed were planted and a whole young leaf harvested from each plant and lyophilized for DNA sampling.
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CEO of Professional Science Editing for Scientists @ prosciediting.com