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The first, coming from our lab, identified ITCH as a WW domain-containing protein that regulates the stability of LATS1 using WW domain arrays.
To verify that the interaction between the two proteins was mediated by a WW domain PY motif interaction, we expressed His6-tagged versions of the four WW domains of human Nedd4 and incubated equal amounts of the fusion proteins (on Ni2+ beads) with GST PY and GST Pro as a control.
The structure determined by the authors shows that the docking of the substrate to a WW domain attached to the HECT domain places steric constraints on the distance of the ubiquitylated lysine from the WW docking site.
Nedd4 consists of an N-terminal C2 domain, three or four WW domains and the ubiquitin ligase HECT domain and, in many cases, engages in interactions with its substrates via a WW domain PY motif interaction [ 4– 9].
A WW II Army veteran.
He was a WW II veteran of the Navy.
She was also a WW II history buff.
Abner, a WW II veteran, spent his career in business with his brothers in real estate.
He was a WW II veteran and alumnus of New York University.
He was a WW II Navy veteran and was with McGraw-Hill Inc. for many years.
Born in NYC, Freddy was a WW II veteran who served five years mostly in the Pacific.
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