The existence of this transcript in a closely related species to the rhesus macaque provides further evidence indicating that the rhesus draft assembly likely contains a contig in the wrong orientation in this region.
Tubes were turned upright for 2 min, and then the 15 min sedimentation was repeated to allow cubes initially settled in the wrong orientation a chance to reorient and bind.
Therefore, a dsDNA that binds to a RecA filament in the wrong orientation cannot establish the correct orientation required for homology recognition and basepairing without full dissociation and rebinding.
Given that flipping does not occur, we conclude that a dsDNA that binds to a RecA filament in the wrong orientation cannot establish the correct orientation without full dissociation and rebinding.
All these lines are homozygous viable or complement a null allele or a deletion, showing that an intronic MiMIC inserted in the wrong orientation does not significantly disrupt gene function.
The remaining 79 lines could either not be mapped because they were insertions into repetitive sequence or were intronic insertions in the wrong frame (13 lines), the wrong orientation (six lines) or within an intron annotated as a UTR (nine lines).
The quadrupolar nature of a gravitational wave will cause a compressing-and-stretching of mutually perpendicular arms, but if a wave comes in at exactly the wrong orientation (in the detector's blind spot), the signal will be missed.
All amplified libraries show an increased proportion of reads in the wrong orientation when compared with the unamplified counterparts (Table 2).
Paired end reads (which consisted of two DNA sequences flanking a nonsequenced internal region), that mapped far apart, in the wrong orientation, or to different chromosomes were flagged as "discordant".
