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The phrase "a well solution" is not correct in English
Did you mean "a good solution" or "a well-crafted solution"? You can use a corrected version of this phrase when discussing the quality of a solution to a problem or challenge. Example: "After reviewing all the options, we concluded that a good solution was to implement a new software system."
Prior to crystallization, the protein was diluted with water to 4 mg ml−1 and mixed with a well solution at a 1 1 v/v ratio.
The crystallization of TmPPase:IDP was carried out using the vapour-diffusion method34 in a 1 μl+1 μl drop on a 24-well plate at room temperature using a well solution containing 32% PEG 400, 0.1 M Tris pH 8.5, 0.1 M MgCl2, 0.1 M NaCl and 2 mM DTT.
Crystals of LsrF were grown via the hanging drop method with a well solution of 22% PEG 400, 200 mM MgCl2, 100 mM Tris pH 8.0.
The CaM-CnA-CBD complex (10 mg/ml) was crystallized at room temperature using the hanging drop vapour diffusion method, with a well solution consisting of 30% PEG 400, 200 mM KCl, and 100 mM Tris (pH 8.5).
Our Rrp4-exosome isoform was concentrated to ∼10 mg/mL in a buffer containing 100 mM Tris-HCl pH 8.6, 200 mM Mg Ac 2, and 25 mM (NH4 2SO4), and crystallized by hanging vapor diffusion against a well solution containing 30% PEG4000 with 100 mM Tris-HCl pH 8.6, 200 mM Mg Ac 2.
Single crystals grew overnight and were cryoprotected in a well solution containing 25% ethylene glycol.
Rhombohedral crystals (0.15×0.15×0.1 mm) grew overnight against a crystallization well solution containing 0.5 ml reservoir solution containing 5% PEG 400, 150 mM ammonium sulphate and 100 mM Bis-Tris pH 5.5 (Figure 1C).
After 1 week, native crystals were transferred into a drop containing well solution supplemented with 5% PEG 4000.
Crystals were soaked in a cryoprotectant solution (well solution complemented with 20 25% glycerol) for 1 5 min and then flash-cooled in liquid nitrogen.
Crystals were cryoprotected by quickly passing through a 1 3 ethylene glycol:well solution and flash frozen in liquid nitrogen.
For the hanging drop method, the protein drop is suspended above the well solution from a seal and makes no contact with the crystallisation plate.
