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Comparative real-time PCR was performed using a Stratagene Mx3000 P real-time instrument in a 96 well format.
Another fragmentation method available is adaptive focused acoustics (Covaris), capable of automating fragmentation in a 96 well format.
C8D1A cells (ATCC; Manassas, VA; cat# CRL-2541) were plated at 1×105 per well in a 6 well format prior to transfection.
To achieve this, a 24 well format was adopted.
The QuantiTect SYBR Green PCR Kit (Qiagen) was used in a 384 well format.
BAC end sequencing can be processed in a 96 well format but requires purified DNA template.
We used transwell plates in a 96 well format and plated ~50,000 Mcellsells in the upper well.
One very novel and powerful technical advance is the in vivo optogenetics in a 96 well format.
Reactions were carried out in a 96 well format in the ABI 7700 Sequence Detector (Perkin Elmer/Applied Biosystems, UK).
High temperature denaturing was performed in a 96 well format heat block set to the desired temperature.
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