Before reaching a critical mass and becoming well established, sequence-based data will need to be confirmed by other methods.
There has been a well established debate over the sequence of surgical vascular repair and bone stabilisation.
We could show that Sanger sequencing as a well established tool is a reliable method for BRAF mutation analysis with a limit of detection of 6.6%.
Target capture and deep sequencing is a well established method for validation of large numbers of putative SNVs detected in WGS data [ 24– 26].
The assessment of the statistical significance of the occurrences of a set of strings W in a set of text strings t1,..., t s is a well established procedure for analysis of biological sequences, in particular via z-score functions [ 18].
The best use of this program, however, is in situations where the phylogeny is well established (e.g., where the input sequences are orthologs so that a well established species tree can be used).
A well established and known technique to collect these trace gases is represented by denuder sequence based systems [17 19], but this technique is time consuming and labour intensive.
SELEX is a well established in vitro selection tool to analyze the structure of ligand-binding nucleic acid sequences called aptamers.
We analysed the 7.0 Mb draft genome sequence of the closely related species Pneumocystis carinii infecting rats, which is a well established experimental model of the disease.
The trees produced from samples taken over a number of years in the same geographical location follow a well established pattern sequences from the same year tend to cluster together, sometimes forming a clade with sequences from the year before or the year after [ 3].
Thus, it seems that a well established nucleosome depleted region, the UAS of Gal1 and Gal10, cannot be reproduced by MNase digestion, size selection and deep sequencing due to the systematic sequence-dependent measurement biases.
