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The strongest sequence differences between the mammalian and the yeast proteins are in the 24 C-terminal amino acids of RPS3A, which show a very poor alignment, with large gaps.
This can be compared to a very poor alignment, for example 'disease – sjuk', which with a frequency of 1 will yield a qvalue of 0.0196 derived from 1/(48+3), given that 'disease' occurs in 48 and 'sjuk' in 3 different candidate term pairs.
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For the scenario where reads from alternative splice variants are assembled into a single contig, some reads will "jump" one or more exons, resulting in a region of very poor alignment to the consensus sequence.
Investigation of other proteins with high BLAST scores gave very poor alignment of the primers and no fragments of similar lengths to the observed SSR fragment patterns.
All hemiascomycetous yeast proteins involved in glucan synthesis or its regulation are 3- to 10-fold longer in sequences, and show very poor alignments with SAKL0H22528p (not shown).
It was a very poor performance".
"There came aboard a very poor man.
It makes a very poor prophet.
We played a very poor football game.
A very poor second.
"He came from a very poor family.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com