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"You are a very lowly man, lacking moral fiber".
It was very cheap at first because we were a very lowly club.
Well, when I worked at the publisher DC Thomson in the early 1960s, I was a very lowly journalist.
One of its officers referred to him as "a provincial educationalist", apparently a very lowly title indeed.
"Because he looked so young, I thought he was trying to hide the fact that he had a very lowly job".
As it happens, I did not go to journalism school, went straight to my first newspaper from university and became a (very lowly) foreign correspondent fairly early on in my career.
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But, as he pointed out, he also spoke to Dublin's GAA team – "and they went out and lost heavily to a very lowly-ranked team the following week... So, I'm 50/50 at this stage".
These lamin A targets were mostly very lowly or not expressed (Ct = 28 38), and none changed expression upon loss of lamin A association in NklTAG cardiac mycoytes (Fig. 3a).
The company, which just turned 10 years old last week, has come a long way from its very lowly beginnings making camera straps for surfers.
To remove possible spurious transcripts and very lowly expressed transcripts, a modified version of RSEM (RNA-Seq by Expectation Maximization [ 52]) available with the Trinity package was applied, and transcripts with an FPKM (fragments per kilobase of exon per million fragments mapped) less than 0.1 were removed.
Briefly, following the approach of Hackett et al. [ 24], genes with an average RPKM value across samples below 0.125 were considered as very lowly or not expressed, genes with an average RPKM value between 0.125 and 1 were considered lowly expressed, between 1 and 10 medium expressed, and above 10 highly expressed.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com