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There are at least two obvious explanations for observing this disagreement: insufficient mixing and a very low sampling fraction.
Other families such as IS91 for example show a very low sampling effort with only twenty sequences present in ISFinder, mainly in a single bacterial clade (alpha Proteobacteria).
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As the reciprocal best hits technique could result in a very low sample size, we bias the requirement of "best hit" in an appropriate direction and also relax the definition of "best hit" vis-à-vis the "top hit".
Furthermore, these previous studies [ 40- 42] used a very low sample size compared to this study.
Apparently a very low sample size in MAXVAR sampling allows outlier hospitals to impact the model estimates more heavily, leading to relatively high prediction errors.
For example, the knowledge parameter would show a large effect size which would result in a very low sample size whereas, self-efficacy would require a lager sample size.
If we would want true scattered sampling from genetically differentiated populations in this species, we would have to be content with a very low sample size, in this case, three.
In a previously published pilot study on this topic, the conjoint analysis approach was shown to be both feasible and functional even in a very low sample size (n=20) [ 54].
However, it should be considered that the value for HeR of one diploid population (Hellberg) was calculated from a very low sample size of individuals carrying an R genome (Table 1).
The above results indeed demonstrate that radiolabelled anti-CD4 mAbs may provide a valuable tool for early and specific diagnosis of the inflamed joints, which could be superior to 99mTc-HIG or 99mTc-HDP scan, but in this case also the available data is limited by a very low sample size.
Genotype (11 versus 12 versus 22) was considered the independent factor and if there was a very low sample size (n = <5) in the homozygous rare allele group, the genotypes were combined into carriers of the rare allele (12 and 22) versus non-carriers of the rare allele (11) to increase the power.
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