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As seen in Figure 2 and supplemental table 4, 18 miRNAs had a varied expression pattern between group 1 and group 3. Seventeen miRNAs were upregulated at least 2-fold with only miR-1914 downregulated 0.5-fold.
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This varied expression pattern is caused by a dl gradient.
As a consequence, Figure 2 has been generated without class selection bias only using the genes that have the most varied expression pattern.
The discriminative binding profiles between sexes should have a natural tie with the varied expression patterns of DmelOBP99b, which calls for more experiments, guiding the behavior of males and females especially during the copulation.
The existence of these varied expression patterns implies a complex set of regulatory mechanisms operating at the RNA level to control the abundance of transcripts encoded by nuclear genes.
If TMAs are to be used for high-throughput biomarker validation in the future, an ability to identify proteins exhibiting such varied expression patterns would be extremely helpful and avoid the reporting of potentially biased data.
Axonemal dyneins display more varied expression patterns but still show an increase of genes with an Arch expression pattern, with 60% of genes in Arch, 27% in Stag, 8% in Pulse, and 5% in Hump (Table S4).
These varied expression patterns suggest that there was a bias toward selective and transcriptional inactivation of some homoeoalleles.
OSK genes displayed varied expression patterns with some OSK genes being strongly and widely expressed such as OSK1, OSK8, OSK11, OSK20 and OSK23.
All DEGs involved in amino acid degradation were up-regulated, while DEGs involved in amino acid synthesis showed varied expression patterns.
Among GSTs that acrylamide up-regulated more than two-fold, those we studied displayed spatially varied expression patterns.
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