Sentence examples for a validation screen from inspiring English sources

Based on our finding of MAGE mutations in the melanoma cell line samples, a Validation Screen was undertaken in which we sequenced the same MAGE genes in 111 fresh tumor samples collected from 86 melanoma patients who had undergone surgical intervention at the Melanoma Clinic at Austin Health, Melbourne, Australia (Table 2).

The work was divided into a discovery screen and a validation screen.

No additional mutations were identified in a validation screen of 19 carcinomas, giving a final frequency of 4.7 % in MOTs.

In all four cases, a primary and a validation screen were performed, making a total of eight screens.

After the p-value was determined by comparing the tumor and naïve samples, the five most significant p-value lysate pools with high intensity with tumor sera, the most significant p-value lysate pools with low intensity with tumor sera, and the five least significant p-value lysate pools were used in a validation screen.

A second validation screen with siRNAs distinct from the primary screen confirmed 29 proteins that gave a MUC5AC hyposecretory phenotype and 5 with a hypersecretory secretory defect (Table 1 ).

Through our analysis of the genome-wide dataset and a secondary validation screen analyzing staining of MSL1 and MSL2, we identified 59 dsRNAs that caused a reproducible SN phenotype (Table S2).

To further study the degree of sensitivity or resistance of each deletion strain in our phenotypic screen, the IC50 for each deletion strain was analyzed in a secondary validation screen and the target strains were arranged based on their degree of sensitivity to NiSO4.

Luciferase intensity was normalized by comparison with plate median in the primary screen and by comparison with a control siRNA in the validation screen (Figure-2).

Thus, for these two particular RNAi screens, we can use this validation screen as an independent experimentally derived reference set to estimate the performance of the NePhe scoring system in identifying FPs.

In the validation screen, the 1,149 genes were sequenced in a further 24 breast and 24 colorectal cancers with matched normal DNA, representing an additional 77 megabases of sequencing.