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The PERMP is a validated, time sensitive, skill adjusted math test that measures attention in ADHD.
The embryos will be cultured in Vitrolife IVF media in a validated time lapse incubator (Embryoscope, Unisense, FertiliTech, Denmark) in 5% CO2, 5%O2 and at 37°C.
Mice were culled by cervical dislocation after a validated time of 5 min. To visualize the soft palate and nasopharynx to pharynx orifice, the mandible and tongue were dissected and photographed in a supine position to allow a ventral view of the palate using a dissection microscope (Leica MZFiii) and Leica DFC300 camera with fluorescence.
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Overall the correlation of RT-PCR and microarray results was 84%, with only 7 of the 45 validated time points not showing the same profile between the microarrays and the RT-PCR.
Birth Rate Plus (BR+) is a validated, real-time objective measure of the number of midwives required to provide a high-quality maternity service at any point in time on delivery suite.
Albumin excretion rates (AERs) were calculated using urinary albumin levels from at least two validated timed sample collections.
In addition, we gathered information on demographic variables, and used the validated timed up-and-go test to measure functional mobility [ 30].
We used the validated time-dependent three-dimensional chemistry transport model European Air Pollution Dispersion chemical transport model (EURAD-CTM) (Ebel et al. 1997; Hass et al. 1993; Memmesheimer et al. 2004; Schell et al. 2001) to predict daily mass concentrations of PM10 (≤ 10 μm in aerodynamic diameter) and PM2.5 on a horizontal grid resolution of 1 km.
In a few cases when an item of assessment could not be validated or was marked according to an arbitrary marking scheme, we applied a school's standard marks per minute ratio to determine a suitable raw mark based on a validated length of time for the assessment item in question.
NGAL was measured using a validated in-house Time-Resolved Immuno-flourometric assay (TRIFMA).
IGFBP-2 was determined by a validated in-house time-resolved immunofluorometric assay (TR-IFMA) as previously described [ 15].
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