(a) +, experimentally validated function; °, in silico predicted function.
For this purpose a validated transfer function is used by the SphygmoCor® system (version 7.0, Atcor Medical, Sydney, Australia) [ 14].
For lack of a validated risk function in secondary prevention, we selected the SCORE function for 10-year cardiovascular risk as the primary outcome in our secondary prevention trial.
Therefore, the functional prediction of miRNA-122 target genes in rainbow trout is consistent with the development and maintenance of a hepatic phenotype, similar to the experimentally validated function of miRNA-122 in mammals [ 6- 8], and more recently, zebrafish [ 5, 29].
The central aortic waveform was derived from the peripheral arterial waveform using a validated transfer function [32,33].
Radial artery waveforms were obtained with a high-fidelity micromanometer (SPC-301; Millar Instruments, Houston, Tex) from the wrist, and a corresponding central waveform was generated with a validated transfer function (Sphygmocor; AtCor Medical, Sydney, Australia) [12], [13], [15].
A corresponding averaged central pressure waveform was then generated, using a validated transfer function.
Central blood pressure was derived from radial pressure waveform by means of a validated transfer function and averaged on three measurements.
The central arterial waveform was derived from 20 sequential waveform records obtained from the peripheral radial artery using a validated transfer function (13).
