Response of rHA binding to immobilized fetuin (red line) or asialofetuin (blue line) was measured following (A) the usual protocol (no chemical blocking) or (B) treatment of the plate with 200 mM ethanolamine in borate buffer, pH 9.5, after the immobilization step (blocking).
They compared their outcome with that of 126 patients treated in a standard "as usual" protocol.
Today, genuflecting to bishops is usually not a part of the usual protocol.
Instead, in a departure from the usual protocol of lengthy deliberations followed by an elaborate competition, the High Museum simply selected Renzo Piano, the Italian architect, who is known to be as subtle and understated as Mr. Meier is imposing.
In a change from its usual protocol, the normally secretive committee also announced that part of its inquiry would be held in public.
In a change from its usual protocol, the normally secretive committee announced that part of its inquiry would be held in public.
Reporters allowed to follow the candidate noticed her abrupt departure, but the campaign refused to answer questions about where she was going or why; in a breach of the usual protocol, her motorcade left without them.
It is a far cry from Ellen DeGeneres's 1997 Time magazine cover, or even the usual protocol for a star, which Mr. Healy describes in this way: Their publicist or agent might inform a reporter that the A-lister was ready to answer the question (often a subject of rumors) of whether he or she was gay.
Because of Mr. Santini's former prominent role in their party, some leading Democrats ignored the usual protocol by which a candidate gets a day free of criticism in which to make his announcment.
In addition to her usual protocol of instituting a routine and enforcing a bedtime, Ms. Frost brought in an expert to discuss Paige's language skills.
As we have demonstrated in a recent study ([ 61], this issue), the surface-adhered PS in microplates without cells can even exert considerable phototoxic effects after addition of PS-free cells in a range similar to the usual protocol where cells are seeded first and the PS is added subsequently.
