The phrase "a universal sequence of" is correct and usable in written English.
It can be used when referring to a series or pattern that is applicable or relevant across different contexts or situations.
Example: "The researchers proposed a universal sequence of steps to follow for effective problem-solving in various disciplines."
Alternatives: "a global series of" or "a standard pattern of".
Influenced by the evolutionary theses of Charles Darwin and Herbert Spencer and by Thomsen's three-age system, Morgan later proposed a universal sequence of cultural evolution in his book Ancient Society; or, Researches in the Lines of Human Progress from Savagery Through Barbarism to Civilization (1877).
The PCR reaction was carried out with three primers, including a locus specific primer extended with a universal sequence of 17 nucleotides (5'- GaCCGGCAGCAAAATTG-3'), a reverse locus specific primer and a universal primer of 17 nucleotides that was 6-Fam, Hex or Tet 5'-labelled (MWG AG Biotech, Ebersberg, Germany).
Author's response: Calling the TOL a universal sequence tree in much of the current literature would have addressed many of our concerns as well, but your arguments have convinced us TOC is more precise in several places.
The marked variability of mixed siliciclastic-carbonate sequences makes the definition of a universal sequence stratigraphic model impossible, as the composition and geometries of systems tracts may change considerably, and sequence stratigraphic surfaces and facies contacts may vary in terms of occurrence and physical expression.
The internal part of these probes is a universal sequence that allows for simultaneous amplification of all circularized, captured sequences prior to massively parallel sequencing.
This has made it problematic to identify clearly these contacting segments as part of a universal sequence block alignable across both Bacteria and Archaea [ 3].
In the present work, the same primer and probe sequences were used except that a universal sequence was added to the 5'-end of the primers according to the principle of HANDS [10].
The structure of the primers composing the cocktail includes (from 5' to 3'): a portion of a universal sequencing primer, e.g. M13 (partial universal primer, PUP), a 'read start' portion (RS), and a portion complementary to the region flanking the target sequence (cocktail portion, CP).
The two universal tags (Cy5-TTACGTGATGGTAATAGTGCTG and Cy3-TTAGGTCGTAGGTGCGTTAGAT) labeled with different fluorescent dyes, were designed to perfectly match their corresponding universal sequences of the SNP probes to form a "sandwich" architecture with the PCR product.
The strong similarity between in vitro C. elegans models and our previously published "Zhang et al". in vitro model trained on yeast DNA [ 22, 27] (Table 1, Figure 2A) indicates that our computational approach can be used to extract universal sequence determinants of nucleosome occupancy regardless of the origin of DNA used in the nucleosome assembly experiments.
Forward primer sequences are labeled with a 5′ universal sequence to allow fluorescent tagging of amplicons during PCR, and reverse primer sequences (with the exception of GC1-R, GC6-R, GC7-R, and GC10-R) are labeled with a 5′ GTTT pigtail sequence for consistent genotyping.
