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Mature mRNAs are generated by trans-splicing of a short-capped RNA, called a spliced leader, which is transcribed from a widely repeated genetic unit via a unique polymerase II external promoter.
Importantly, the RdRp of two +ssRNA viruses (Thosea asigna virus and Euprosterna elaeasa virus) formerly related to the tetraviruses – and recently grouped in a separate family, the Permutotetraviridae (T = 4) [ 13] – shares with the birnavirus VP1 a unique polymerase motif rearrangement that has been found otherwise only in Drosophila A virus and the plant alpha-like virus Grapewine virus Q.
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In addition to these major classes of PTPs, SSU72 is a unique RNA polymerase II CTD phosphatase that shares high sequence similarity with PTPs (Ganem et al. 2003; Zhang et al. 2012).
The unique polymerase chain reaction product was analyzed on an ABI Prism genetic analyzer (Applied Biosystems).
For instance aphidicolin inhibits DNA replication in archaea and eukaryotes but not bacteria by targeting their unique polymerase [ 116, 117].
The distance of 25 bp was initially chosen to allow for the design of unique polymerase chain reaction primers.
Although SMRT sequencing is capable of directly detecting modified nucleotides by their unique polymerase kinetics without bisulfite conversion [ 32], the introduction of an amplification step abolishes its ability to detect any epigenetic modifications of template DNA.
By using a unique bacteriophage DNA polymerase, [Oslash]29 DNA Polymerase, that has an intrinsic high processivity, we are able to achieve significant amplification within 1 hour at 35[deg ]C.
In plastids, transcription is under the control of two types of RNA polymerases, a unique eubacterial-type plastid-encoded polymerase (PEP) and multiple phage-type nucleus-encoded polymerases (NEPs) (reviewed in [ 27] and [ 28]).
In the same way, the genomes of the Firmicutes clade have a unique heterodimeric DNA polymerase III α-subunits, PolC and DnaE [ 54].
A unique high fidelity DNA polymerase PrimeSTARTM HS DNA Polymerase (TaKaRa, Osaka, Japan) was used in PCR.
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