Sentence examples for a unique alignment to from inspiring English sources

Average percent identity was approximately 92%, with an average alignment length of 384 bp, and 51,087 contigs (91%) produced a unique alignment to a single chromosome (Table S6).

For TIC splicing, a threshold of 8 nt yielded a unique alignment to a TIC target in about 1 in 30,000 reads.

We considered a read to be evidence for an exon-exon junction if it had a unique alignment to a target with zero or one mismatch and no indels and if the alignment extended past the midpoint of the target by a certain overhang amount.

We then aligned the Illumina reads to the D. melanogaster exome, requiring unique alignment to a single location and allowing one, two, or three mismatches.

Overall, the average percent identity was approximately 92%, with an average alignment length of 306 bp, and 17,084 contigs (93%) produced one unique alignment to a bovine chromosome (Table S2).

Sequence lengths generally range from 25 50 bp (short sequences), which is sufficient for unique alignment to a reference genome [ 6].

The ULCS approach for determining aneuploidy requires only that the reads are sufficiently long to enable unique alignment to the genome.

Since reads generated from the alternative allele overlapping a differentiating site will have at least a single base mismatch to the reference genome, we successively allowed one (-v 1), two (-v 2), or three (-v 3) mismatches, but still required unique alignment to the reference genome (-m 1).

2D reads with a unique alignment (UA) match to a genomic location were retained for further analysis.

Each aligned read was assigned a mapping quality that indicates whether the read has a unique alignment or can be aligned to multiple positions in the genome [ 22].

Except for the Oo library, for which one mismatch was allowed due to the longer reads, only those reads with a unique alignment and zero mismatch to the reference genome were used in further analysis.