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This paper proposes a two-step methodological framework that takes into account the diversity of farms in the prototyping of new CMS.
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Open image in new window Fig. 9 Effective stress distribution with various steps a 34th step; b 64th step; c 84th step; d 104th step Open image in new window Fig. 10 Variation in effective stress with various steps.
Open image in new window Fig. 7 Distribution of the velocity field at different steps during the cold forging process a 34th step; b 64th step; c 84th step; d 104th step Open image in new window Fig. 8 Variation in velocity at various steps in various deformation regions.
This prospective methodological study was undertaken as a two-step process.
For example, β/α-barrel fold is present in Fmd/Fwd subunit A (catalyzing the 1st step in the pathway) and Mer (5th step); Ferredoxin fold – in Ftr (2nd step) and in the other [Fe-S] cluster-containing enzymes, such as McrA and Fmd; Rossmann fold – in the Hmd enzyme (4th step).
We thus found a subset of pathways enriched from DEGs and, starting from a matrix containing mean of genes for each pathway (3rd step), we created a matrix score for each pair of pathways (4th step).
4th step: finding a pair peak.
We ran five independent chains for 50,000 steps each and recorded samples from the posterior distribution every 20th step following a 30,000 step burn-in.
These nuclei act as a base for the generation of new pores during 2nd step.
A search strategy, each 10000 steps long using ten short chains, sampling every 20th step, followed by ten long chains each of 20000 steps sampled every 20th step, gave consistent results among runs and was used in all analyses.
In order to establish a more efficient method for VSEL purification from UCB, we employed a three-step isolation strategy based on removal of erythrocytes by hypotonic lysis (1st step), followed by immunomagnetic separation of CD133+ cells (2nd step), followed by FACS-based isolation of CD133+Lin−CD45− cells (3rd step).
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