The phrase "a two step protocol" is correct and usable in written English.
You can use it when referring to a process or set of procedures that involves two distinct stages or steps.
Example: "To ensure security, we will implement a two step protocol for user authentication."
Alternatives: "a dual-step procedure" or "a two-phase protocol".
The analysis was carried out with a two step protocol starting with 20 seconds at 95°C, followed by 40 cycles of 1 second at 95°C and subsequent 20 seconds at 60°C.
The analysis was carried out with a two step protocol starting with 20 seconds at 95°C, followed 40 cycles of 1 second at 95°C and subsequent 20 seconds at 60°C.
Basta and Hammer [ 48], established a two step protocol to isolate C3: PEG precipitation and fast protein liquid chromatography (FPLC) Mono Q ion exchange chromatography, using human and guinea pig.
This was a two- step protocol using the materials and methods included with the DuraScript RT-PCR Kit (Sigma Aldrich).
This notion is supported by a two-step protocol.
Immunohistochemical staining of paraffin sections was carried out using a two-step protocol.
Illumina MiSeq paired-end indexed amplicon libraries were prepared following a two-step protocol.
We utilized this chemistry for site-specific fluorescence labeling of proteins on the cell surface and inside living mammalian cells by a two-step protocol.
Biggers JD, Racowsky C. The development of fertilized human ova to the blastocyst stage in KSOM AA) medium: is a two-step protocol necessary?
In this work, monodisperse PdCu@PtCu NCs with various shell thicknesses and compositions have been synthesized through a two-step protocol.
Pd/Au hollow cone-like microstructures (HCMs) have been electrodeposited on indium tin oxide (ITO) using a two-step protocol, which involves the nucleation pulse and succedent constant potential reduction in the presence of metal precursors and polyvinylpyrrolidone (PVP).
