After being defrost and adapted to the cultivation for five passages, cells were cultivated by more than a hundred passages in RPMI-1640, supplemented with 13% heat-inactivated fetal calf serum (Nutricell, Campinas, SP, Brazil) and 25 μg/mL streptomycin.
At the bottom of a window, two passages from Scripture were taped to the glass.
Both NG and HG cells were subcultured for at least a further two passages in their respective medium before being used for experimentation.
With an additional two passages, the prevalence of this population decreased to 27.1% (Figure 2H), and the prevalence of other subpopulations increased.
Brain slices' adhesion to glass slides was improved by a two minutes passage in the microwave oven.
Here Darwin, this time not citing Lyell by name, in his note "(a)," harks back to two passages in Lyell's second volume (see also Hodge 1983).
Next, a series of 50 passages at a rate of two passages per day was performed by inoculating 20 μl of culture into 5 ml fresh LB broth and incubating at 37 °C without shaking.
Virus detection (Table 1) was performed by IHC, M gene RRT-PCR (PCR), and virus re-isolation in 9 11 day old embryonated fowls' eggs [32] (two eggs per sample for each passage, with a total of two passages).
Cells were subjected to a maximum of two passages in culture before transfection.
The cells were disrupted with a high-pressure homogenizer (two passages of 1000 bar).
A pre-test/multiple posttest design was used with three measures: ability to interpret passives correctly, ability to produce passives at the sentence level, ability to reconstruct a three sentence passage in which passives were embedded.
