The phrase "a two fold cutoff" is not standard in written English; it should be "a twofold cutoff." You can use it when referring to a cutoff point that has two distinct aspects or criteria.
Example: "The study implemented a twofold cutoff to ensure that only the most relevant data was included in the analysis."
Alternatives: "a dual cutoff" or "a bifold cutoff."
Adding 1 nM androgen only adds 232 differentially expressed genes (two fold cutoff, 133 down regulated and 101 up regulated).
To our surprise, the comparison of MOCK-transfected PC3 cells with AR-transfected cells showed 3,452 genes with differential expression (two fold cutoff) even without addition of androgens (i.e. in ethanol control) (Table S1 and Figure 1).
A surprising finding was that the comparison of MOCK-transfected PC3 cells with AR-transfected cells identified 3,452 differentially expressed genes (two fold cutoff) even without the addition of androgens (i.e. in ethanol control), suggesting that a ligand independent activation or extremely low-level androgen activation of the AR.
We showed that AR could be activated in low androgen or ligand independent manner in advanced prostate cancer cell line PC3 cells expressing the AR. the comparison of vector-transfected PC3 cells with AR-transfected PC3 cells showed that 3,449 genes are differentially expressed (two fold cutoff) even without addition of androgens (Figure 1 and table S1).
Ask how you could make a ten fold, hundred fold, thousand fold or greater difference through social media.
Thus, only genes with a change fold cutoff > 2 were considered to be significantly differentially regulated.
A > |1.5| fold cutoff and P ≤ 0.05 was used.
These 697 probe sets were queried for those that pass the 1.3 fold cutoff in all three cross comparisons of at least one permutation of three cross comparisons (Fig. 2, cyan and red dots).
Cutoffs of two fold were set for up-regulation (blue) or suppression (red) after light pulse, and the number of probesets that satisfy each cutoff was plotted for each point.
In addition to the ON and OFF categories, we also investigated transcripts that are constitutively expressed at all stages (N = 22,257) but are differentially expressed between at least two developmental stages using a two-fold cutoff for significance.
