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To simulate in vivo human PC, MatLyLu cells were transfected with a truncated sequence of human HER2/neu cDNA cloned into the pCI-neo vector.
Supplemental Table S2 presents a truncated sequence of a CLIP domain serine protease expressed in An. darlingi SGs, 86% identical to the An.
Using a long RNA avoids the biases that are often introduced when a truncated sequence of an RNA is studied and ensures that important structural regions are not omitted.
To further characterize the role of HIF-1 α/MGMT axis in prompting GBM cell resistance to TMZ therapy, we transfected TMZ-sensitive GBM cells (derived from the peripheral layers of the tumour) with a plasmid bearing a truncated sequence of HIF-1 α (Hif-1 α-ΔODD) lacking the oxygen-dependent degradation (ODD) domain.
In some experiments, GBM cells derived from the peripheral layers of the mass were transfected using a protocol for transient transfection of adherent cells with Effectene reagent (Qiagen, Hilden, Germany) with a pcDNA3.1 backbone bearing a truncated sequence of the HIF-1 α transcript named HIF-1 α-ΔODD or a control pcDNA3.1 empty plasmid.
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The latter protein, however, is present only as a truncated sequence in our set of opossum proteins, lacking 8 amino acids.
All Drosophila melanogaster recombinant constructs, with the exception of CL mutants, were derived from a truncated sequence, comprising the first 559 AA of the Drosophila kinesin-1 heavy chain (KHC) with eGFP and a 6xHis-tag engineered at the C-terminus, hereafter referred to as DmK-WT (Shastry and Hancock, 2010; Milic et al., 2014).
It is noteworthy that during an effort to identify genes involved in tumour progression, an N-terminal truncated sequence of GADD34 was identified by subtractive hybridisation from a rat embryonic cell line (Su et al, 1997).
The first crystal structure of CPR was obtained using an N-terminally truncated sequence of the rat isoform.
We also found that the truncated sequence of miR-1 can form 3'-ESS while the original sequence can not.
Additional file 2 provides for the full as well truncated sequence of 25 such enzymes, which includes two sequences previously described during the analysis of the G. m. morsitans fat body library [ 29], as well as the analysis of the proventriculus cDNA library [ 80].
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