Degradation of PrP by lichen extracts in infected BH samples or PrPTSE-enriched preps differs from digestion by PK in that PK cleaves an N-terminal portion of the PrPTSE molecule, leaving a truncated end-product, whereas lichen extracts can reduce PrP immunoreactivity to below the limit of immunoblotting detection.
We observed many incomplete ORFs interrupted by a sequence gap between contigs: 39 ORFs had a truncated terminal at one end and 124 ORFs (62 pairs) had internal gaps.
When you come to a screeching halt at the truncated end of the southbound 710 Freeway just below Colorado you know something is wrong.
The primers were designed to provide an overlap with the data obtained from TSPs1 3, and hence confirm that the sequence retrieved was an extension of the previous mRNA and not a new variant with a truncated 3′ end.
The primers were designed to provide overlapping data and hence confirm that each of the sequences retrieved was an extension of the previous mRNA and not a new variant with a truncated 3′ end.
In particular, there were three lines of evidence that rps3, which was previously annotated as a pseudogene because of a truncated 5′ end, is actually functional as a protein-coding sequence.
The MREs of some miRNAs that target PTEN, including miR-19b and miR-20a, are preserved in the truncated 3' end of the PTENP1 transcript, which allow it to act as a miRNA target decoy for PTEN.
A distinguishing characteristic of the NGFR construct from the other MaxiP constructs was the presence of a truncated gene at the end of the BAC that is adjacent to the HPRT/ Hprt locus.
Bovine GRK5-H6 was expressed in a truncated form ending at residue 561, followed by a hexahistidine tag.
For APC, estimating 'overexpression' was more problematic, as SW480 cells do not accumulate wild-type APC1 instead they accumulate a truncated APC1 ending at amino acid 1338.
This issue was more complex in the case of APC, as SW480 cells do not accumulate wild-type APC1 – instead they accumulate a truncated APC1 ending at amino acid 1338.
