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The relative amount of kakusei transcript in the MBs and optic lobes (OLs) was quantified by real-time reverse transcription-polymerase chain reaction (RT-PCR) and plotted against the waggle-phase duration of the dances, which were video-recorded and analysed using a transparent observation hive (Fig. 2).
Mice were placed into a transparent observation chamber (30 × 30 × 25 cm) for adaptation 30 min before the experiments (Kim et al., 2001).
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To exclude the possibility that the neural activity in the re-orienting bees is due to the increased light exposure when they leave the hive, a similar experiment was performed using re-orienting bees from transparent observation hives (Figure 6G and H).
Experimental colonies were housed in two-frame transparent observation hives containing approximately 4000 bees.
A formalin solution (1% in 0.9% saline, 50 μl/paw) was injected into the hind paw plantar surface of rats (n = 6) (i.pl)., and the animals were individually placed in transparent observation chambers, as previously described [ 19, 20].
The as-purchased, optical-quality qPCR tube caps, with 3 mm diameter, transparent observation windows, are not optimized for protein adsorption; antibodies, therefore, were covalently attached by the following method.
That attraction opened two years before these Chicago extensions and promised to be far more spectacular: a transparent-floored observation deck stretching outward 70 feet from the canyon's rim and suspended 4,000 feet above the Colorado River (as high as 900 Oprahs and an impossible number of deep-dish pizzas).
These mice were individually placed in a transparent plexiglass cage observation chamber (25 cm × 15 cm × 15 cm).
He once constructed a transparent beehive for scientific observation, and made an exquisitely detailed model of the moon.
Gap junctional communication was examined by electroporating the fluorescent dye Lucifer yellow into cells grown on a transparent electrode, followed by observation of the migration of the dye to the adjacent, non-electroporated cells under fluorescence illumination.
Nonetheless, observation through a transparent material such as glass is a very distinctive way of directly measuring deformation [3, 4].
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Justyna Jupowicz-Kozak
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