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However, a transit peptide at the N-terminus of PtTPS12 suggests a plastidal localization and thus an in vivo function as monoterpene synthase in accordance with the absence of PtTPS12 sesquiterpene products in the poplar volatile blend.
It is conceivable that mitochondrial intermediate peptidase (M03.006), which removes a transit octapeptide from the N-terminus of proteins synthesized in the cytoplasm but destined for the mitochondrial matrix, may have a preference as far away from the cleavage site as S8 (31).
Of the missing 83 residues in the N-terminus, region 1−59 is a transit peptide (7).
Each thioesterase contains a transit peptide of variable sequence at the N-terminus that signals their import into the chloroplast, thus only the mature protein sequences were considered in our analyses (starting at L88 in AtFatB).
To demonstrate that the general net positive charge of a transit peptide but not a specific aa residue at the N-terminus of plastid-destined precursor is crucial for import, we replaced the glutamate residues with arginine residues in a second round of mutagenesis.
Consistent with this prediction, the amino acid sequence alignment showed that CmsHSP2 possessed an ∼30 amino acid extension at the N-terminus that was absent in other sHSPs that may function as a transit peptide (fig. 3 A).
A transit hub is in the works.
Or vote to finance a transit system.
"It's a new M line," a transit employee shouted.
A transit rider.
Arbat is a transit camp.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com