Sentence examples for a transformation efficiency of from inspiring English sources
The second round was performed with Top 10 cells that have a transformation efficiency of 10 transformants/μg of plasmid DNA.
Electrocompetency has been established in Thermotoga in this study, and a transformation efficiency of ~2 transformants per μg of DNA has been observed in RQ7 and ~0.25 in T. maritima.
Puromycin-resistant clones transformed by the vectors appeared after transfection with a transformation efficiency of approximately 1,300 per 1.6 × 106 electroporated cells, irrespective of the vector differences.
A total of 23 PCR positive events were obtained with a transformation efficiency of 5% around.
The minimum inhibitory concentration of benomyl against C.militaris was 1.5 μg/ml and a transformation efficiency of 7 colonies/μg DNA was obtained by the protoplast-PEG method with the vector pBT6, containing the benomyl resistance gene.
The selectable marker, together with an enhanced green fluorescent protein (EGFP) gene, was delivered into C. vulgaris protoplasts by using a PEG-mediated method, giving a transformation efficiency of 356 ± 30 cfu per μg vector DNA.
The manufacturer gives a transformation efficiency for these cells of 10 10 transformants/μg of plasmid DNA.
In conclusion, with the optimized approach, a high transformation efficiency of 145 transformants per 10 conidia was obtained.
Hygromycin-resistant clones transformed by the vectors appeared with a common transformation efficiency of approximately 1,500 per 1.6 × 106 electroporated cells.
In addition, yeasts are attractive hosts for the high throughput screening of peptides or proteins from large combinatorial libraries (Chao et al. 2006; Li et al. 2007; Ueda 2004), with a high transformation efficiency of plasmids of up to 1 1.5 × 108 transformants/μg achieved (Benatuil et al. 2010).
Glass bead-mediated delivery displayed a high transformation efficiency of C. reinhardtii with CrPDS-L505F, demonstrating clearly that the engineered endogenous CrPDS is a dominant selectable marker for C. reinhardtii and possibly for other green algae.
